In your own words, explain why it is necessary to include only one chain-terminating/synthesis-terminating nucleotide in each well of the electrophoresis instrument.

 

 Determine the DNA sequence by analyzing the location of each nucleotide across the four lanes of the electrophoresis instrument. Provide the DNA sequence as your answer here.

 

Important: remember that your sequence starts from the shortest fragment!

 

What is the significance of finding that DNA sequences are highly similar for two different species?

 

 Below, align the newly determined DNA sequence with the sequences of the three known plant species (see data under the Activity Form tab). Next, compute the percent alignment of the bases for the three comparisons