3A. List the protein sequence for your candidate gene plus the five other homologous protein sequences for each organism into a single text file in FASTA format (/6)
3B. We are asking you to conduct a BLAST-p search for this tutorial, but there is also an option to perform BLAST-n search. BLAST-n investigates DNA sequences (i.e. genes). What differences would you expect to see in terms of sequence conservation between DNA and protein sequences? (HINT: think about protein translation) (/2)
3C. Each BLAST result is given a series of scores: E-value, Score, and Percent Identity (%). Define each term (/1.5)
3D. Prepare Table I (/3), reporting the E-value, Score, and Percent Identity (%) for each of the five other homologous protein sequences. When preparing the table caption, include relevant information for understanding the table.
3E. Which homolog was most similar to your organism’s gene of interest? (/0.5)