Temperature and Enzyme Activity

 

1. Using Ex 3, record the relative color intensity at five-minute intervals to determine the temperature range for catechol oxidase. Record Step 6 data in row 1, Step 7a data in row 2, Step 7b data in row 3

Time

(min) Tube 1

4oC Tube 2

23oC Tube 3

40oC Tube 4

60oC Tube 5

80oC Tube 6

100oC

1 0 Yellow YELLOW  YELLOW YELLOW CLEAR TO MILKY CLEAR TO MILKY

2 5 YELLOWISH ORANGE YELLOWISH ORANGE YELLOWISH ORANGE ORANGE CLEAR TO MILKY CLEAR TO MILKY

3 10 YELLOWISH ORANGE (2) BROWNISH RED ORANGE (5) BROWNISH RED (4) ORANGE (3 )  CLEAR TO MILKY ( 0 ) CLEAR TO MILKY (0 )

 2.Construct a bar graph of the data for just the ten-minute readings. Label the axes, Y axis is the 1-5 intensity scale and X axis is temperature. Give the graph a title and provide units.  Sign, date and prepare an image of your graph and include it with this lab report.  

 3.What is the optimal temperature for catechol oxidase activity?  37 degrees celsius  

 4.The enzyme is denatured at which temperatures? Because enzymes are proteins, they are denatured by heat. Therefore, at higher temperatures (over about 55°C